9261s  (Cell Signaling Technology Inc)

Journal: International Journal of Molecular Sciences

Article Title: Pro-Inflammatory Macrophage Phenotype Skewing Induced by Tumor Treating Fields (TTFields)

doi: 10.3390/ijms262412086

Figure Lengend Snippet: TTFields activate GEF-H1 and drive macrophage transcriptional reprogramming via c-Jun and p65. BMDMs were polarized to either an M1 state (IFN-γ + LPS) or an M2 state (IL-4) and then exposed to TTFields. ( A , B ) Western blot analysis of phosphorylated GEF-H1 (Ser886) following TTFields treatment at 15, 30, and 60 min in M1 and M2 macrophages. ( A , C ) Phosphorylation of NF-κB p65 (Ser536) in BMDMs treated with TTFields. ( A , D ) Phosphorylation of c-Jun (Ser63) in TTFields-treated macrophages. Densitometric analysis for phosphorylation fold change is shown as mean ± SEM; N ≥ 3. * p < 0.05, and ** p < 0.01; one-way ANOVA followed by Dunnett test compared with time zero.

Article Snippet: phosphorylated c-Jun (Ser63) , 1:500 , 9261 , Cell signaling *.

Techniques: Western Blot, Phospho-proteomics

Journal: Cell Death Discovery

Article Title: Atorvastatin improves spermatogenesis in murine and in vitro human chronic orchitis models through restoring blood-testis barriers

doi: 10.1038/s41420-025-02749-6

Figure Lengend Snippet: a Venn diagram of differential expressed proteins (DEPs) between groups. b Enriched GO terms of the co-downregulated proteins in Vehicle and T + AT groups compared with TNF-α group. c Heatmap showing the top 10 co-downregulated proteins according to the fold change between T + AT group and TNF-α group. d Western blot images of MMP3 and MMP9 in TM4 Sertoli cells from Vehicle, TNF-α and T + AT groups. e Grayscale value analysis of western blot for MMP3 and MMP9 in TM4 Sertoli cells from Vehicle, TNF-α and T + AT groups, n = 3. f , g Immunofluorescence of MMP3 and MMP9 (green) co-stained with SOX9 (red) in Vehicle, EAO-Sal and EAO-AT groups and quantitative analysis for immunofluorescence intensity of MMP3 and MMP9 in SOX9 + Sertoli cells from 20 randomly selected fields. SOX9 labeled Sertoli cells. Scale bar, 10 μm. n = 4 testis samples per group. h Western blot assay of Rac1, cJUN and Phospho-cJUN (p-cJUN) in TM4 Sertoli cells from Vehicle, TNF-α and T + AT groups. i Grayscale value analysis of western blot for Rac1 and p-cJUN in TM4 Sertoli cells from Vehicle, TNF-α and T + AT groups, n = 3. j Immunofluorescence of cJUN (green) co-stained with SOX9 (red) in Vehicle, EAO-Sal and EAO-AT groups and quantitative analysis for immunofluorescence intensity of cJUN in the nucleus of SOX9 + Sertoli cells from 20 randomly selected fields. SOX9 labeled Sertoli cells. Scale bar, 10 μm. k Western blot images of MMP3, MMP9, CX43 and ZO1 in TM4 Sertoli cells after T-5224 treatment. Data are presented as mean ± SEM, * P < 0.05, ** P < 0.01, **** P < 0.0001, ns indicates no statistical significance.

Article Snippet: The primary antibodies used in this study were listed: rabbit anti-HMGCR (A19063, ABclonal, 1:1000), rabbit anti-CX43 (26980-1-AP, Proteintech, 1:1000), rabbit anti-ZO1 (21773-1-AP, Proteintech, 1:1000), rabbit anti-CTNNB1 (ab32572, Abcam, 1:1000), rabbit anti-MMP3 (17873-1-AP, Proteintech, 1:1200), rabbit anti-MMP9 (10375-2-AP, Proteintech, 1:1200), rabbit anti-cJUN (ab32137, Abcam, 1:4000), rabbit anti-p cJUN (9261S, Cell Signaling Technology, 1:1000), rabbit anti-Rac1 (24072-1-AP, Proteintech, 1:1000).

Techniques: Western Blot, Immunofluorescence, Staining, Labeling